WebDec 13, 2007 · This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived … WebNov 22, 2024 · A biolabile disulfide-containing linker (LSSL) was incorporated into siRNA strand at the final stage of solid-phase oligonucleotide synthesis through corresponding non-nucleotidic phosphoramidite with subsequent activation of the hydroxyl group of the linker by DSC and interaction with trileucine or cholesteryl-6-aminocarbamate, giving …
Chemistry and Synthesis Center (CSC) NHLBI, NIH
WebAll SignalSilence ® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. ... Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. WebGlen-Pak™ Reverse Phase Oligonucleotide ... or 5-Me-C derivatized solid supports can contain high molecular weight ... as well as those requiring mild deprotection. It is also compatible with the synthesis of RNA and siRNA. Another important advantage of a universal support is that it reduces the need to keep inventory of many ... phim the wicker man
Solid‐Phase Synthesis of Sequence‐Defined T‐, i‐, and U‐Shape …
WebDNA Oligonucleotide Synthesis. Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for … WebJan 1, 2014 · To develop carriers for cytosolic and systemic siRNA delivery, our laboratory has recently developed a sequence-defined polymer platform compatible with solid-phase … WebMar 1, 2008 · Assessment of 4-nitrogenated benzyloxymethyl groups for 2'-hydroxyl protection in solid-phase RNA synthesis. Cieślak J, Kauffman JS, Kolodziejski MJ, Lloyd … phim the witcher 2019