Phosphinothricin-resistant
WebPlant resistance to phosphinothricin is conferred through the bar gene, and due to the fast degradation of phosphinothricin in soil and in water, it presents a low risk for environmental contamination. Product Specifications Phosphinothricin Glufosinate or Glufosinate ammonium Also known as Bayer’s Basta TM Formula: C 5 H 15 N 2 O 4 P WebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related …
Phosphinothricin-resistant
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WebNov 6, 2006 · Selection for kanamycin-, phosphinothricin- and hygromycin B-resistance commonly takes 7-10 d and high seedling density and fungal contamination may result in failure to recover transformants. Results: A method for identifying transformed seedlings in as little as 3.25 d has been developed. WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis.
WebA bioassay was developed for identifying transgenic wheat plants based on De Block et al.'s [8] ammonium-multiwell assay which allows qualitative and quantitative evaluation of the expression of the enzyme phosphinothricin acetyl transferase (PAT). Important parameters in the assay are the use of young leaf tissues, short incubation period (6 h) and a high … WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1.
WebSep 12, 2015 · In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus reporter and a bar … WebA gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase.
WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ...
WebAug 21, 2024 · After 14 days of incubation at 30 °C, carboxin-resistant transformants were screened for phosphinothricin-resistance protein and GUS expression. The transformants expressing opbar were screened on CYM plates with 150 mg/L phosphinothricin. the poiltics forumsWebCAS No. 7803-51-2. Phosphine (PH₃) is a colorless, flammable, and explosive gas at room … sideways t symbolWebA bioassay was developed for identifying transgenic wheat plants based on De Block et … the poimandres themeWebOct 23, 2015 · The R295K mutation has also never been previously reported as an … the poignant music of melting ice ny timesWebPhosphinothricin is not an effective antibiotic because of its inability to be readily … sideways tv spot 2004WebOct 4, 2009 · The transgenic plants were resistant to phosphinothricin up to 200 mg/l, … sideways ufoWebNov 25, 2014 · L-phosphinothricin (PPT) is a naturally occurring amino acid with herbicidal activity and it is active component of herbicide "Basta". PPT is a potent inhibitor of glutamine synthase and thus causes rapid increase in ammonia concentration in plants and this leads to death of plant cell. thepoinball meloblox