Fitc偶联annexin-v凋亡检测试剂盒
Web北京义翘神州(Sino Biological, Inc.)自主研发的高品质 Annexin V/7-AAD 凋亡检测试剂盒以 Annexin V 联合 7-AAD/PI 的双染法(提示 ... WebMay 27, 2024 · Annexin V-FITC细胞凋亡检测试剂盒(Annexin V-FITC Apoptosis Detection Kit)是用FITC标记的重组人Annexin V来检测细胞凋亡时出现在细胞膜表面的磷脂酰丝氨酸的一种细胞凋亡检测试剂盒。可以使用流式细胞仪、荧光显微镜或其它荧光检测设备进行检测。
Fitc偶联annexin-v凋亡检测试剂盒
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WebAnnexin V is a Ca 2+-dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS). The ApoDETECT Annexin V-FITC Kit binds to … WebBioLegend
http://www.bio-review.com/fitc-labeling-kit/ Web3. 加入5-15µg纯化的重组Annexin V。注意,不同的细胞系,以及凋亡的不同阶段,其Annexin V位点 饱和所需要的纯化的重组Annexin V含量不同。某些情况下,为达到最好效果,可以减少细胞数量, 0.5´105个细胞加5-15µg纯化的重组Annexin V。 4. 轻轻混匀,室温反应15分钟。 5.
WebAnnexin V是一种分子量为35-36kD的Ca2 + 依赖性磷脂结合蛋白,能与细胞凋亡过程中翻转到膜外的磷脂酰丝氨酸(PS)高亲和力特异性结合。在正常细胞中,磷脂酰丝氨酸(PS)只分布在细胞膜脂质双层的内侧,而在细胞凋亡早期,细胞膜中的磷脂酰丝氨酸(PS)由脂膜 ... WebDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry: Kit Contents: Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X …
WebANNEXIN V- FITC/PI 凋亡检测试剂盒用于细胞凋亡检测,Annexin V 是一种分子量为35.8 KD 的Ca2+依赖性磷脂结合蛋白,能与细胞凋亡过程中翻转到膜外的磷脂酰丝氨酸(Phosphatidylserine, PS)高亲和力特异结合。
Web一般描述. Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞,并使用流式细胞仪进行定量。. AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯(FITC)偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。. 该试剂盒包含碘化丙啶 (PI),用于标记细胞膜完全破坏 … greenwich bank and trust glenvilleWebThe Annexin V-FITC Kit contains reagents for the simultaneous fluorescent detection of apoptotic, necrotic, and dead cells through the specific binding of FITC-labeled Annexin V to phosphotidylserine (PS). The kit consists of Annexin V-FITC, Annexin V Binding Buffer, and Propidium Iodide Solution. fo76 deathclaw gauntlet planWebFITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. fo76 crossbow plan locationWeb悬浮细胞收集:离心5分钟;贴壁细胞:用不含EDTA的胰酶消化收集后(注:胰酶消化时间不宜过长,否则会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合),于室温2000rpm离心5~10分钟,收集细胞;. (3)细胞洗涤:用预冷1×PBS(4℃)重悬细胞一次,2000rpm离心5 ... greenwich bank \u0026 trust company greenwich ctWebAnnexin V-FITC Microscopy Staining Protocol. 1. Wash cells twice with 1X PBS. 2. Wash cells once with 1X Annexin V Binding Buffer. 3. Stain cells with Annexin V-FITC diluted 1:10 in 1X Annexin V Binding Buffer for 15 min at RT. We typically use 2 ml total volume per Labtek II chamber slide (i.e., 200 µl Annexin V-FITC and 1.8 ml 1X Binding Buffer. fo76 demolition expertWebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. greenwich bank of americaWebJul 27, 2024 · 将Annexin V进行荧光素(EGFP、FITC)标记,以标记了的Annexin V作为荧光探针,利用荧光显微镜或流式细胞仪可检测细胞凋亡的发生。碘化丙啶(Propidium Iodide, PI)是一种核酸染料,它不能透过完整的细胞膜,但对凋亡中晚期的细胞和死细胞,PI能够透过细胞膜而使 ... fo76 blue ridge caravan backpack