WebAutophagy was measured by transmission electron microscopy (TEM), immunofluorescence, western blot and immunohistochemistry. Cell viability was detected with MTT and colony formation assay. The apoptosis rate was measured by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). WebJan 28, 2015 · The conclusion that the cytoprotective autophagy induced by radiation has been converted to cytostatic autophagy by the inclusion of 1,25-D 3 or EB 1089 is strongly supported by complementary genetic silencing studies, where the autophagy-related genes BECN1 and ATG5 were knocked down utilizing shRNA, as shown in the immunoblots in …
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WebDec 16, 2015 · What distinguishes cytostatic autophagy from the cytoprotective form is the failure to detect evidence of cell killing reported in the breast tumor cells . Both Gewirtz and Kroemer's group demonstrated cytoprotective autophagy by radiation alone but the addition of vitamin D or EB 1089 converted cytoprotective autophagy to cytostatic autophagy ... WebJan 28, 2015 · Evidence for cytostatic autophagy by pharmacological inhibition. (A, B) Western blot indicating that pharmacological inhibitors Baf (100 nM, panel A) and … bonanza boxed set
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WebJul 2, 2024 · Autophagy is a self-regulating process in which aggregated proteins and aged (or malfunctioning) organelles are separated by double-membrane … WebAutophagy is also associated with cancer therapies, wherein autophagy has been identified to play four different roles, specifically cytoprotective, cytotoxic, cytostatic, and non-protective functions [Citation 12], although … WebNov 27, 2024 · However, when autophagy is prolonged or strongly overstimulated, Cav-1 will be degraded [65,66,67], ... A proper modulation of Cav-1 content can significantly influence the cell sensitivity to different cytostatic agents. Induced overexpression of Cav-1 in drug resistant cells, ... bonanza bowling alley warren mi